eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2020-09-24 8 9 506 515 10.12691/jfnr-8-9-7 JFNR2020897 article N Nasiruddin 1 2 Minghui Ji 2 2 Zhangxin Yu 2 2 Guangying Chen chgying123@163.com 2 2 Tariq Masood 3 Funing Ma 4 Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, Hainan Normal University, Haikou 571127, P. R. China Department of Agricultural Chemistry, The University of Agriculture, Peshawar Pakistan Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China Millettia speciosa, a traditional Chinese folk medicine, used as immunity enhancer and anti-bronchitis. The study was proposed to investigate phytocomponents and pharmacological activities (antityrosinase, sunscreen and anticancer) of the different fractions of M. speciosa leaves. The sample was extracted with solvents of increasing polarity i.e. petroleum ether (PE), ethyl acetate (EtOAc), methanol (MeOH) and the total flavonoid content for each fraction was determined. The GC-MS analysis revealed various important bioactive compounds. The identified major compounds included beta sitosterol (12.039%), (Z)-9-octadecenamide (6.299%) and gamma sitosterol (4.910%). The highest flavonoid content was recorded for the PE extract i.e. 47.50 ± 0.40%. The PE extract showed significant antityrosinase activity (70.30 ± 0.90 %) with IC50 0.035 mg/ml followed by the MeOH extract i.e. 66.50 ± 1.20% with IC50 0.039 mg/ml. The highest sunscreen activity was noted for the EtOAc extract by observing maximum absorbance value (1.518 ± 0.49) in UVA region followed by 1.214 ± 0.46 in UVB zone. The highest cytotoxicity (29.45 ±1.17% viability) towards MCF-7 breast cancer cells line was noted for the PE extract with IC50 6.73 µg/ml. Overall, our study revealed the presence of potential bioactive compounds for each extract that exhibited significant therapeutic activities and therefore validate the importance of M. speciosa leaves as a source of natural medicine. http://pubs.sciepub.com/jfnr/8/9/7/jfnr-8-9-7.pdf Millettia speciosa GC-MS analysis bioactivities flavonoid sunscreen antityrosinase anticancer