@article{jfnr2020896,
author={{Sun, Jian-Rui and Wang, Da-Hong and Zhao, Jun-Feng and Gu, Shao-Bin},
title={Ultrasonic-assisted Extraction, Purification, Antioxidant and Antibacterial Activity of Polysaccharide from <i>Cornus officinalis</i> Leaves},
journal={Journal of Food and Nutrition Research},
volume={8},
number={9},
pages={496--505},
year={2020},
url={http://pubs.sciepub.com/jfnr/8/9/6},
issn={2333-1240},
abstract={The ultrasonic-assisted extraction process of polysaccharide from <i>Cornus officinalis</i> leaves (COLP) was optimized. After isolation and purification, the antioxidant and antibacterial activities of polysaccharide were studied <i>in vitro</i>. The extraction rate was highest under conditions of ultrasonic power 340 W, solvent to solid ratio 26:1(v/w), extraction temperature 71 ˇăC and extraction time 2.3 h, reaching 7.04%, which was consistent with the model predicted value. The crude polysaccharide was isolated and purified by DEAE-52 cellulose chromatography column, and four components COLP-1, COLP-2, COLP-3 and COLP-4 were obtained. Then, Sephadex G-100 chromatography column was used to purify COLP-1 and COLP-3, and two components COLP-1-1 and COLP-3-2 were obtained. COLP-1-1 had strong scavenging ability against DPPH free radicals, against hydroxyl radical, and superoxide anion radical, with the highest clearance rate reaching 81.48%, 79.48% and 72.87% respectively. COLP-3-2 also had strong scavenging ability against DPPH free radicals, against hydroxyl radical, and superoxide anion radical, with the highest clearance rate reaching 88.57%, 76.64% and 86.08% respectively. The results indicated that COLP-1-1 and COLP-3-2 had higher antioxidant activity, and the antioxidant activity of COLP-3-2 was stronger than COLP-1-1. The?results of antibacterial activity showed?that COLP-1-1 had the highest antibacterial activity on <i>Bacillus subtilis</i> and weaker antibacterial activity on<i> Escherichia coli</i>,<i> Salmonella typhimurium </i>and<i> Staphylococcus</i><i> </i><i>aureus</i>; COLP-3-2 had a certain antibacterial activity on <i>Escherichia coli</i>,<i> Pseudomonas aeruginosa</i>,<i> Salmonella typhimurium </i>and<i> Bacillus subtilis</i>.},
doi={10.12691/jfnr-8-9-6}
publisher={Science and Education Publishing}
}