eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2020-07-29 8 7 313 319 10.12691/jfnr-8-7-2 JFNR2020872 article Ching-Chih Liu 1 Jen-Yin Chen 2 3 Chin-Chen Chu 3 Shih-Ying Chen 4 Heuy-Ling Chu 5 Pin-Der Duh ipdduh@mail.cnu.edu.tw 5 Department of Ophthalmology Chi-Mei Medical Center, 710402, Taiwan, R.O.C. Department of Senior Citizen Service Management, Chia Nan University of Pharmacy and Science, 71710, Taiwan, R.O.C. Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, 71710,Taiwan, R.O.C. Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, 71710,Taiwan, R.O.C. The aim of this study is to investigate the effect of chlorogenic acid (CA) on 3T3-L1 adipocytes and its mechanism action. CA at 1-25 ¦ÌM showed no cytotoxicity to 3T3-L1 pre-adipocytes and 3T3-L1 adipocytes. CA significantly inhibited oil red O-stained material (OROSM) and intracellular triglyceride levels in 3T3-L1 adipocytes in a concentration-dependent manner. In addition, CA down-regulated the glycerol-3¨Cphosphate dehydrogenase (GPDH) and peroxisome proliferator¨Cactivated receptor ¦Ã (PPAR¦Ã) activity. A real-time polymerase chain reaction revealed that CA inhibited the PPAR¦Ã, CCAAT/enhancer-binding protein alpha (C/EBP¦Á), sterol regulatory element binding protein-1c (SREBP-1c), and fatty acid synthase (FAS) gene expression, which may in part account for anti-adipogenesis of CA. Thus, CA could act as a potential lipid lowering functional resource. http://pubs.sciepub.com/jfnr/8/7/2/jfnr-8-7-2.pdf chlorogenic acid adipocytes anti-adipogenesis lipid accumulation transcription factor