@article{jfnr2020852,
author={{Wibowo, Indra and Nasution, Fauzi Ramadhani and Taufik, Intan and Zain, Roya Suffah and Marlinda, Nissa and Utami, Nuruliawaty and Wardiny, Putri Yunitha and Putra, Ramadhani Eka and Tan, Marselina Irasonia and Sumarsono, Sony Heru},
title={Anti-inflammatory Activity of Indonesian Propolis in Zebrafish (<i>Danio rerio</i>) Larvae},
journal={Journal of Food and Nutrition Research},
volume={8},
number={5},
pages={225--230},
year={2020},
url={http://pubs.sciepub.com/jfnr/8/5/2},
issn={2333-1240},
abstract={One candidate for anti-inflammatory agents, polyphenol-rich Indonesian propolis, has been rarely studied. This study was conducted to confirm the presence of its anti-inflammatory activity. Zebrafish larvae, as a model, were divided into four groups consisting of a control group, a lipopolysaccharide (LPS)-treated group, an LPS-treated group followed by treatment in propolis solution for 24 hours, and a propolis-treated group. Myeloid leukocytes migrating into the intestine and intestinal goblet cells were counted. The expression level of pro-inflammatory (<i>tnf-¦Á, il-1¦Â, il-8, </i>and<i> il-6</i>) and anti-inflammatory (<i>il-10</i>) cytokine genes were determined using quantitative reverse transcription polymerase chain reaction (RT-PCR). It was shown that Indonesian propolis administration to LPS-induced zebrafish larvae resulted in reduced myeloid leukocytes in the intestine, increased intestinal goblet cells, and decreased the expression level of <i>tnf-¦Á </i>(<i>P</i>&lt;0.05). Overall, these results suggest that Indonesian propolis could be a potential agent to protect against inflammatory damage<b>.</b>},
doi={10.12691/jfnr-8-5-2}
publisher={Science and Education Publishing}
}