eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2020-06-11 8 5 225 230 10.12691/jfnr-8-5-2 JFNR2020852 article Indra Wibowo indra@sith.itb.ac.id 1 Fauzi Ramadhani Nasution 1 Intan Taufik 1 Roya Suffah Zain 1 Nissa Marlinda 1 Nuruliawaty Utami 2 Putri Yunitha Wardiny 2 Ramadhani Eka Putra 2 Marselina Irasonia Tan 2 Sony Heru Sumarsono 2 School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Indonesia Research Center for Biotechnology, Cibinong Science Center, Indonesian Institute of Sciences, Bogor, Indonesia One candidate for anti-inflammatory agents, polyphenol-rich Indonesian propolis, has been rarely studied. This study was conducted to confirm the presence of its anti-inflammatory activity. Zebrafish larvae, as a model, were divided into four groups consisting of a control group, a lipopolysaccharide (LPS)-treated group, an LPS-treated group followed by treatment in propolis solution for 24 hours, and a propolis-treated group. Myeloid leukocytes migrating into the intestine and intestinal goblet cells were counted. The expression level of pro-inflammatory (tnf-¦Á, il-1¦Â, il-8, and il-6) and anti-inflammatory (il-10) cytokine genes were determined using quantitative reverse transcription polymerase chain reaction (RT-PCR). It was shown that Indonesian propolis administration to LPS-induced zebrafish larvae resulted in reduced myeloid leukocytes in the intestine, increased intestinal goblet cells, and decreased the expression level of tnf-¦Á (P<0.05). Overall, these results suggest that Indonesian propolis could be a potential agent to protect against inflammatory damage. http://pubs.sciepub.com/jfnr/8/5/2/jfnr-8-5-2.pdf indonesian propolis inflammation cytokine lipopolysaccharide zebrafish