eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2019-08-11 7 8 560 567 10.12691/jfnr-7-8-2 JFNR2019782 article Francisco J Esparza-Martínez fj.esparza@ugto.mx 1 Pablo García Solis 2 Rita Miranda-López 3 Vicente Peña-Caballero 3 Departamento Ingeniería Agroindustrial, Universidad Guanajuato, campus Celaya-Salvatierra, Ing. Barros Sierra No. 201, Ejido de Santa María, Celaya Guanajuato, México Departamento de Medicina, Universidad Autónoma de Querétaro, Clavel No.200, Prados de La Capilla, Santiago de Querétaro, Querétaro. México Posgrado de Ingeniería Bioquímica, Tecnológico de Celaya, Celaya, Gto., México. Av. Tecnológico s/n, Celaya, Gto. México Several epidemiological studies have suggested that phenolics have antineoplastic activities. The objective of this study was to determine the antiproliferative effect of rich phenolics extracts of mandarin (Citrus reticulate) and lime (Citrus aurantifolia) fruit waste in colorectal cancer cells, RKO (carcinom) and HT-29 (adenocarcinom). Antiproliferative effect was evaluated using the methylthiazolydiphenyl-tetrazolium bromide (MTT) assay and testing phenolics extracts from different mandarin and lime fruit waste drying temperatures (fresh, 60, 90, 120ºC), at different times (24, 48 and 72 h). Mandarin and lime phenolics extracts inhibit cell viability of colon cancer cell lines, HT-29 and RKO, at 24 h to 72 h. Results suggested that phenolics extracts had different action mechanisms on each type of cells and therefore, more studies were required to elucidate such mechanisms. http://pubs.sciepub.com/jfnr/7/8/2/jfnr-7-8-2.pdf antiproliferative activity colorectal cancer phenolic compounds