eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2019-04-01 7 4 270 278 10.12691/jfnr-7-4-3 JFNR2019743 article Min Young Kim 1 Gwi Yeong Jang 2 Yoon Jeong Lee 3 Kyung Mi Kim 4 Dae Joong Kim 5 Junsoo Lee 5 Heon Sang Jeong hsjeong@chungbuk.ac.kr 5 Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon, 16429, South Korea Department of Herbal Crop Research, National Institute of Horiticultural and Herbal Science, Rural Development Administration, Eumseong 27709, South Korea Depatment of Food Science and Biotechnology, Chungbuk National University, Cheongju 28644, South Korea National Academy of Agricultural Science, Rural Development Administration, Jeonju 54875, Korea Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, South Korea This investigation aims to evaluate the influence of high hydrostatic pressure (HHP) treatment after germination on soyasaponin conversion and physiological activity in black soybean [Glycine max (L.)]. Black soybean was germinated for 4 days and subjected to 24 h at 0.1-150 MPa; the crude soyasaponin extracts were then evaluated for anti-inflammatory and anti-obesity activity. The highest crude soyasaponin content of 57.85 mg/g was found in germinated black soybean treated at 100 MPa. Soyasaponin Bb and Bc were the major B-group soyasaponins in raw black soybean; their contents in raw and germinated black soybean increased after HHP treatment. The lipid accumulation rate during 3T3-L1 adipocyte differentiation in germinated HHP-treated black soybean ranged from 93.25-65.95% at the concentration of 400 μg/mL. The highest anti-obesity effect was observed after germination and 150-MPa treatment. Black soybean sprout extracts treated at 150 MPa significantly inhibited the lipopolysaccharide-induced expression of inflammatory markers by RAW 264.7 macrophages. http://pubs.sciepub.com/jfnr/7/4/3/jfnr-7-4-3.pdf black soybean sprout high hydrostatic pressure soyasaponin anti-inflammatory activity anti-obesity activity