eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2018-09-18 6 9 557 560 10.12691/jfnr-6-9-3 JFNR2018693 article Jongsung Lee 1 See-Hyoung Park shpark74@hongik.ac.kr 2 Department of Integrative Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea Department of Bio and Chemical Engineering, Hongik University, Sejong, 30016, Korea The anti-oxidant activity of daidzein was accessed by checking the expression level of superoxide dismutase 2 (SOD2) in HK-2 cell, a kidney proximal tubular cell line. SOD2 expression was increased in HK-2 cells treated by daidzein with a dose and time-dependent manner. Daidzein reduced the reactive oxygen species (ROS) level (about 60% compared to DMSO control with p = 0.005) in HK-2 cells. Daidzein caused FOXO3 to be translocated from cytosol into nucleus. Down-regulation of FOXO3 by transfection of siRNA against FOXO3 attenuated the expression of SOD2 and ROS level (p = 0.006) by daidzein. Taken together, this study has shown that daidzein displays anti-oxidant activity in HK-2 kidney proximal tubular cell line through up-regulation of FOXO3/SOD2 signaling pathway. http://pubs.sciepub.com/jfnr/6/9/3/jfnr-6-9-3.pdf daidzein anti-oxidant HK-2 FOXO3 SOD2