@article{jfnr2018663,
author={{Isingoma, Barugahara Evyline and Samuel, Mbugua and Edward, Karuri},
title={Determination of the Minimum Inhibition Concentration of Moringa <i>oleifera</i> Leaf Powder against Some Common Diarrhoea Causing Pathogens},
journal={Journal of Food and Nutrition Research},
volume={6},
number={6},
pages={365--369},
year={2018},
url={http://pubs.sciepub.com/jfnr/6/6/3},
issn={2333-1240},
abstract={The antimicrobial properties and nutrient content of Moringa <i>oleifera</i> leaves have made them of great interest in current scientific research. This study determined the Minimum Inhibition Concentration of Moringa <i>oleifera</i> leaf powders against some common diarrhoea causing bacteria. Four bacterial strains of Escherichia <i>coli</i>, Salmonella <i>typhi</i>, Shigella <i>shiga</i> and Staphylococcus <i>aureaus</i> were used in the study. Moringa <i>oleifera </i>leaves powders were thoroughly mixed with sterile Brain Heart Infusion (BHI) broth and centrifuged for 15minutes before being strained using a filter paper. Ten millilitres of sterilized BHI with different levels of moringa leaf powders were each inoculated with 0.05 millilitres of standardised suspension of tested bacteria. Sterilized BHI alone and BHI with varying levels of moringa leaf powder without the pathogens were also prepared to be used in standardising the spectrophotometer. Readings were taken before and after incubating the different samples and bacterial growth was tested by measuring optical density in the broth at 600 nm after incubation for 24 hours at 35ˇăC. The difference in spectrophotometer readings before and after incubation of the samples was used to indicate growth of bacterial pathogens. Results indicated that Moringa oleifera leaf powders inhibited growth in all the four bacterial strains due to its antimicrobial properties. The minimum inhibition concentration of Moringa <i>oleifera</i> leaf powder against 0.05 millilitres of standardised Escherichia <i>coli</i> varied between 8.4g to 9.8g of moringa <i>oleifera</i> leaf powder per 100 millilitres of BHI broth while for Staphylococcus <i>aureaus</i> it ranged between 9.8g to 10.2g Moringa <i>oleifera</i> leaf powder per 100mls of BHI broth. The results of this investigation can be used to guide low income households and pharmacists on the quantities of dried M. <i>oleifera</i> leaf powder to use in an effort to eliminate diarrhoea due to E. <i>coli</i> and S. <i>aureaus </i>pathogens.},
doi={10.12691/jfnr-6-6-3}
publisher={Science and Education Publishing}
}