eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2018-12-28 6 12 773 784 10.12691/jfnr-6-12-8 JFNR20186128 article Jung-Ha Lee 1 Jae-Bong Lee 2 Jin-Tae Lee 2 Hae-Ryoun Park 2 Jin-Bom Kim jbomkim@pusan.ac.kr 2 Pusan National University, South Korea Daegu Haany University, South Korea Bromelain is obtained from Ananas comosus and is of significant clinical interest. Many studies have demonstrated bromelain's ability to treat immune-mediated conditions including reduction of platelet aggregation, angina pectoris and surgical trauma. ROS elimination were evaluated using a variety of anti-oxidation effect tests containing DPPH assay, ABTS+ radical scavenging assay, hydrogen peroxide scavenging assay. Western blotting analysis and RT-PCR were used to investigate an anti-inflammatory agent in LPS-induced RAW cells. Treatment with bromelain exhibited significant scavenging abilities on superoxide radicals compared with control. As results of analyzing iNOS and COX-2 band appearance according to bromelain treatment, it inhibited the band expression dose-dependently. Also bromelain inhibits the phosphorylated-MAP kinases signaling pathways. Furthermore, it reduced c-Fos, phosphorylation of c-Jun and c-Jun. According to the results, it is demonstrated that bromelain of Ananas comosus extract have significant anti-inflammatory ability as well as antioxidant ability. http://pubs.sciepub.com/jfnr/6/12/8/jfnr-6-12-8.pdf bromelain anti-oxidant effect anti-inflammatory effect public oral health