eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2018-12-19 6 12 725 730 10.12691/jfnr-6-12-2 JFNR20186122 article I Gusti Ayu Nyoman Danuyanti danuyanti@gmail.com 1 2 Arta Farmawati 3 Sunarti 3 Doctoral Program, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia Department of Biochemistry, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia Hypertriglyceridemia causes hypoxia and increases the release of free fatty acids in adipose tissue through lipolysis which stimulates an increase in TLR4 and NFκβ levels as well as TNF-α and IL-6 secretion. The high fiber diet had a suppressing effect against TLR4 and NFκβ gene expression and decreased TNF-α and IL-6 levels. This study evaluated the effect of high fiber diet (P1= 1.04 g content of fiber/rat/day, P2= 2.07 g content of fiber /rat/day and P3= 3.11 g content of fiber/rat/day) on TNF-α and IL-6 levels as well as expression of TLR4 and NFκβ in rats induced by high fat and fructose diet for 6 weeks. The findings indicate that the administration of high fiber diet to the hypertriglyceridemia rats could control body weight gain and significantly reduce TNF-α and IL-6 levels (p<0.05) lower than the hypertriglyceridemia groups when compared before treatment. In addition, the treatment of high fiber diet resulted in suppressing the expression of TLR4 and NFκβ gene (p<0.05) when compared with the normal and hypertriglyceridemia groups. The suppression of TLR4 and NFκβ were correlated with TNF-α and IL-6 levels. In conclusion, the administration of the high fiber diet to the hypertriglyceridemia rats was able to decrease TNF-α and IL-6 levels possibly by suppression of TLR4 and NFκB gene expression. http://pubs.sciepub.com/jfnr/6/12/2/jfnr-6-12-2.pdf TLR4 NFʦʦ TNF-α IL-6 hypertriglyceridemia high fiber diet