eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2017-07-07 5 7 510 517 10.12691/jfnr-5-7-9 JFNR2017579 article Haneul Jo 1 Ok-Kyung Kim 1 2 Ho-Geun Yoon 3 Eungpil Kim 4 Kyungmi Kim 5 Yoo-Hyun Lee 6 Kyung-Chul Choi 7 Jeongmin Lee 8 Jeongjin Park pjj8425@hanmail.net; wjjin@chonnam.ac.kr 8 8 Woojin Jun pjj8425@hanmail.net; wjjin@chonnam.ac.kr 8 8 Division of Food and Nutrition, Chonnam National University, Gwangju, South Korea Department of Biochemistry and Molecular Biology, Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, Seoul, South Korea Marine Biotechnology Research Center, Wando, South Korea Department of Biofood Analysis, Korea Bio Polytechnic, Ganggyung, South Korea Department of Food and Nutrition, The University of Suwon, Suwon, South Korea Department of Biomedical Sciences and Department of Pharmacology, University of Ulsan College of Medicine, Seoul, South Korea Department of Medical Nutrition, Kyung Hee University, Yongin, South Korea In the present study, the protective effects of 10% ethanol extract of Capsosiphon fulvescens (CFE10) against alcoholic liver damage were investigated in vitro using CYP2E1-overexpressing hepatocytes (HepG2/2E1). To determine whether CFE10 attenuated ethanol-induced cell death, we compared the viability of HepG2/2E1 cells treated with 250 mM ethanol in the presence or absence of CFE10. Cell viability significantly increased after treatment with CFE10 and ethanol compared with that of cells treated with only ethanol. Additionally, CFE10 inhibited ethanol-induced ROS formation and lipid peroxidation. We also found that CFE10 attenuated the mRNA expression of CYP2E1, as well as decreased ethanol-induced lipid droplets, through stimulation of the AMPK pathway. Based on these results, the protective effect of CFE10 extract from C. fulvescens against liver damage and fatty liver induced by ethanol may occur via the alleviation of oxidative stress. http://pubs.sciepub.com/jfnr/5/7/9/jfnr-5-7-9.pdf capsosiphon fulvescens alcohol CYP2E1 reactive oxygen species liver damage