eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2017-05-25 5 6 406 412 10.12691/jfnr-5-6-7 JFNR2017567 article Ning-Jo Kao 1 Zwe-Ling Kong kongzl@mail.ntou.edu.tw 1 Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan. R.O.C. In previous study, we found curcumin to possess anti-inflammatory properties in lipopolysaccharide (LPS)-induced macrophage cells due to the involvement of curcumin and S-nitrosylation in the NF-ʦB pathway. However, the role of curcumin on regulation of NF-ʦB signaling pathway through S-nitrosylation in an in vitro inflamed human intestinal mucosa model has not yet been elucidated. This study aimed to concern inhibitory effects of curcumin on NF-ʦB pathway in two type of inflamed human intestinal cells, Caco-2 and HT-29. Western blot presented the protein expression of iNOS can be reduced by treated curcumin with 30 μM for 12h. Consistently, pro-inflammatory cytokines, such as IL-1β, IL-6, TNFα and IFN-γ was also repressed. The results also shows curcumin reduced the amount of nitrite and nitrate in inflamed human intestinal cells, Caco-2 and HT-29, maintained total S-nitrosylation level on proteins. Furthermore, the protection of S-nitrosylation on IKKæ in inflamed Caco-2 and HT-29 cells by curcumin caused the repression of I¦B phosphorylation and NF-ʦB activation. In conclusion, this study verified that curcumin-mediated S-nitrosylation may be as an important regulator for anti-inflammation in an in vitro inflamed human intestinal mucosa model. http://pubs.sciepub.com/jfnr/5/6/7/jfnr-5-6-7.pdf curcumin S-nitrosylation inflamed human intestinal cells NF-ʦB IʦB IKK nitric oxide