@article{jfnr2017567,
author={{Kao, Ning-Jo and Kong, Zwe-Ling},
title={Curcumin Suppresses the Activity of Inhibitor-百B Kinase in an<i> in vitro Inflamed Human Intestinal Mucosa Model</i> by <i>S</i>-nitrosylation},
journal={Journal of Food and Nutrition Research},
volume={5},
number={6},
pages={406--412},
year={2017},
url={http://pubs.sciepub.com/jfnr/5/6/7},
issn={2333-1240},
abstract={In previous study, we found curcumin to possess anti-inflammatory properties in lipopolysaccharide (LPS)-induced macrophage cells due to the involvement of curcumin and <i>S</i>-nitrosylation in the NF-百B pathway. However, the role of curcumin on regulation of NF-百B signaling pathway through<i> S</i>-nitrosylation in an <i>in vitro inflamed human intestinal mucosa model </i>has not yet been elucidated. This study aimed to concern inhibitory effects of curcumin on NF-百B pathway in two type of inflamed human intestinal cells, Caco-2 and HT-29. Western blot presented the protein expression of iNOS can be reduced by treated curcumin with 30 米M for 12h. Consistently, pro-inflammatory cytokines, such as IL-1汕, IL-6, TNF汐 and IFN-污 was also repressed. The results also shows curcumin reduced the amount of nitrite and nitrate in inflamed human intestinal cells, Caco-2 and HT-29, maintained total <i>S</i>-nitrosylation level on proteins. Furthermore, the protection of <i>S</i>-nitrosylation on IKK汕 in inflamed Caco-2 and HT-29 cells by curcumin caused the repression of I百B phosphorylation and NF-百B activation. In conclusion, this study verified that curcumin-mediated <i>S</i>-nitrosylation may be as an important regulator for anti-inflammation in an <i>in vitro inflamed human intestinal mucosa model</i>.},
doi={10.12691/jfnr-5-6-7}
publisher={Science and Education Publishing}
}