@article{jfnr2017566,
author={MIN, Jin Gi},
title={Purification and Characterization of Anti-complementary Polysaccharides from <i>Teucrium viscidum</i>},
journal={Journal of Food and Nutrition Research},
volume={5},
number={6},
pages={399--405},
year={2017},
url={http://pubs.sciepub.com/jfnr/5/6/6},
issn={2333-1240},
abstract={The anti-complementary activities of aqueous polysaccharides from <i>Teucrium viscidum</i> and their inhibitory effects on the complement system were examined. TM4a, a polysaccharide purified by Sepharose CL-6B, showed significant anti-complementary activities on the classical, alternative and lectin pathways. The predominant linkages of TM4a consisted of 3.1 mol of 4-O-linked galactose, 2.8 mol of 3-O-linked galactose and 2.1 mol of 4-O-linked glucose; the branched polysaccharide was also indicated by the presence of 3,6-O-linked and 2,6-O-linked glucose. The inhibitory effects of TM4a and heparin showed similar results with regard to all complement pathways, except at the concentration of 100 ¦Ìg/mL. After blocking the complement components with TM4a (620 ¦Ìg/mL), C3 component, C3-deficient serum, or C3-deficient serum with a C3 component were added to the mixture. Hemolysis of the mixture with the C3 component, or C3-deficient serum showed about 18% or 21% hemolysis of EA cell, while the mixture with the C3-deficient serum containing C3 showed an increase in the hemolysis of EA cells of 68% with regard to the classical pathway. Regarding the alternative pathway, C3 component with TM4a was not cleaved into C3b and C3a during electrophoresis.},
doi={10.12691/jfnr-5-6-6}
publisher={Science and Education Publishing}
}