@article{jfnr2017561,
author={{Kim, Kui-Jin and Lee, Jin-Ha and Lee, Boo-Yong and Lee, Ok-Hwan},
title={Optimized <i>Cirsium setidens</i> Nakai Fermented by <i>Lentinula edodes</i> Attenuates Lipid Accumulation by Regulating Fatty Acid Oxidation-mediated Lipolysis in 3T3-L1 Cells and High Calorie Diet-induced Obese Zebrafish},
journal={Journal of Food and Nutrition Research},
volume={5},
number={6},
pages={354--361},
year={2017},
url={http://pubs.sciepub.com/jfnr/5/6/1},
issn={2333-1240},
abstract={<i>Cirsium setidens</i> Nakai is an edible herb. Previously we found that fermented <i>Cirsium setidens</i> Nakai (FCSN) has a large amount of major bioactive compound compared to <i>Cirsium setidens</i> Nakai. In this study, we aimed to examine the anti-obesity effect of FCSN using 3T3-L1 cells <i>in vitro</i> and high calorie diet-induced obese (HDIO) zebrafish model <i>in vivo</i>. Our results demonstrated that FCSN significantly inhibited intracellular lipid accumulation in 3T3-L1 cells. FCSN was shown to reduce the expressions of crucial adipocyte differentiation markers, including PPAR¦Ã and aP2. FCSN also decreased the production of ROS due to the up-regulated expressions of SOD1, SOD2, GPx, and catalase. Furthermore, we observed that FCSN also altered the levels of energy metabolism and ¦Â-oxidation-associated genes such as AMPK, ACC, and CPT-1. In addition, ATGL, a key lipolysis enzyme, was stimulated while the differentiation of 3T3-L1 was suppressed by FCSN. Strikingly, we found that FCSN dramatically increased both the energy metabolism and ¦Â-oxidation associated genes and subsequently prevented the increase of body fat accumulation in high calorie diet-induced obese zebrafish. Taken together, this is the first study that demonstrates that FCSN has the beneficial activity to suppress adipogenesis in 3T3-L1 cells and ameliorate an obese-associated health condition <i>in vivo</i>.},
doi={10.12691/jfnr-5-6-1}
publisher={Science and Education Publishing}
}