eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2017-05-13 5 6 354 361 10.12691/jfnr-5-6-1 JFNR2017561 article Kui-Jin Kim 1 Jin-Ha Lee 2 Boo-Yong Lee 2 Ok-Hwan Lee loh99@kangwon.ac.kr 2 Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam, Gyeonggi, Republic of Korea Department of Food Science and Biotechnology, Kangwon National University, Chuncheon, Kangwon, Republic of Korea Cirsium setidens Nakai is an edible herb. Previously we found that fermented Cirsium setidens Nakai (FCSN) has a large amount of major bioactive compound compared to Cirsium setidens Nakai. In this study, we aimed to examine the anti-obesity effect of FCSN using 3T3-L1 cells in vitro and high calorie diet-induced obese (HDIO) zebrafish model in vivo. Our results demonstrated that FCSN significantly inhibited intracellular lipid accumulation in 3T3-L1 cells. FCSN was shown to reduce the expressions of crucial adipocyte differentiation markers, including PPARγ and aP2. FCSN also decreased the production of ROS due to the up-regulated expressions of SOD1, SOD2, GPx, and catalase. Furthermore, we observed that FCSN also altered the levels of energy metabolism and æ-oxidation-associated genes such as AMPK, ACC, and CPT-1. In addition, ATGL, a key lipolysis enzyme, was stimulated while the differentiation of 3T3-L1 was suppressed by FCSN. Strikingly, we found that FCSN dramatically increased both the energy metabolism and β-oxidation associated genes and subsequently prevented the increase of body fat accumulation in high calorie diet-induced obese zebrafish. Taken together, this is the first study that demonstrates that FCSN has the beneficial activity to suppress adipogenesis in 3T3-L1 cells and ameliorate an obese-associated health condition in vivo. http://pubs.sciepub.com/jfnr/5/6/1/jfnr-5-6-1.pdf fermented Cirsium setidens Nakai 3T3-L1 adipogenesis zebrafish β-oxidation high fat diet