@article{jfnr2017554,
author={Kang, Hyun Woo},
title={Anti-inflammatory Effects of Extract from <i>Eucommia ulmoides</i> Oliv. Leaves on Macrophage Cells},
journal={Journal of Food and Nutrition Research},
volume={5},
number={5},
pages={309--312},
year={2017},
url={http://pubs.sciepub.com/jfnr/5/5/4},
issn={2333-1240},
abstract={This study investigated the anti-inflammatory effects of <i>Eucommia ulmoides</i> Oliv. (EUO) leaf extracts on RAW264.7 macrophages. Cell toxicity was determined by MTT assay. We evaluated the anti-inflammatory effects of EUO extracts by measuring nitric oxide (NO), inducible nitric oxide synthase (iNOS) production, and cyclooxygenase-2 (COX-2) expression by western blotting. EUO ethanolic extracts (0.1, 1, 5, and 10 ¦Ìg/mL) significantly suppressed LPS-stimulated production of NO. EUO ethanolic extracts reduced the expression of iNOS and COX-2 proteins. The present results show that EUO ethanol extract has potent anti-inflammatory effects on RAW264.7 macrophages. These results also suggest that the anti-inflammatory effects of EUO extracts may be related to the inhibition of LPS-stimulated NO production. Therefore, ethanolic extracts of EUO leaves may be utilized as a good source of functional food and pharmaceutical applications for protection against inflammatory diseases.},
doi={10.12691/jfnr-5-5-4}
publisher={Science and Education Publishing}
}