eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2017-03-20 5 3 187 190 10.12691/jfnr-5-3-8 JFNR2017538 article Yeon-Joo Lee 1 Ji-Hyun Hwang 1 Kui-Jin Kim Kuijin.Kim@cha.ac.kr, bylee@cha.ac.kr 1 Boo-Yong Lee Kuijin.Kim@cha.ac.kr, bylee@cha.ac.kr 1 Department of Food Science and Biotechnology, CHA University, Gyeonggi 463-400, South Korea In the present study, we examined the beneficial effect of Ecklonia cava-derived polysaccharide (ECAP), which is residual product after bioactive polyphenol isolation form Ecklonia cava, on neurotoxicity and oxidative stress in hydro peroxide-treated human microglia HMO6 cells. We sought that ECAP inhibited the expression of p53 and Bcl-2 in hydro peroxide-induced HMO6 cells. ECAP dramatically suppressed the cleaved form of caspase-3 in hydro peroxide-treated HMO6 cells. Moreover, we observed that hydro peroxide stimulated the expression of glucose-6-phosphate dehydrogenase (G6PDH) and its downstream target NADPH oxidase 4 (NOX4) in HMO6 cells. In contrast, ECAP suppressed the expression of G6PDH and NOX4 in hydro peroxide-treated HMO6 cells. We also observed that anti-oxidant enzymes including superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), and catalase were stimulated by ECAP to protect the neurotoxicity in hydro peroxide-treated HMO6 cells. Taken together, we demonstrated that ECAP may inhibit hydro peroxide-mediated neurotoxicity through the regulation of p53 and Bcl-2 genes in HMO6 cells and ameliorate oxidative stress in hydro peroxide-treated HMO6 cells. Therefore we suggest that ECAP may hold the potential to prevent the incidence of neuronal damage-mediated neurodegenerative diseases. http://pubs.sciepub.com/jfnr/5/3/8/jfnr-5-3-8.pdf Ecklonia cava seaweed polysaccharide neurotoxicity oxidative stress microglia HMO6 neuroprotection