@article{jfnr2017538,
author={{Lee, Yeon-Joo and Hwang, Ji-Hyun and Kim, Kui-Jin and Lee, Boo-Yong},
title={<i>Ecklonia cava</i>-derived Polysaccharide Prevent Hydro Peroxide-induced Oxidative Stress and Neurotoxicity in Human Microglial HMO6 Cells},
journal={Journal of Food and Nutrition Research},
volume={5},
number={3},
pages={187--190},
year={2017},
url={http://pubs.sciepub.com/jfnr/5/3/8},
issn={2333-1240},
abstract={In the present study, we examined the beneficial effect of <i>Ecklonia cava</i>-derived polysaccharide (ECAP), which is residual product after bioactive polyphenol isolation form <i>Ecklonia cava</i>, on neurotoxicity and oxidative stress in hydro peroxide-treated human microglia HMO6 cells. We sought that ECAP inhibited the expression of p53 and Bcl-2 in hydro peroxide-induced HMO6 cells. ECAP dramatically suppressed the cleaved form of caspase-3 in hydro peroxide-treated HMO6 cells. Moreover, we observed that hydro peroxide stimulated the expression of glucose-6-phosphate dehydrogenase (G6PDH) and its downstream target NADPH oxidase 4 (NOX4) in HMO6 cells. In contrast, ECAP suppressed the expression of G6PDH and NOX4 in hydro peroxide-treated HMO6 cells. We also observed that anti-oxidant enzymes including superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), and catalase were stimulated by ECAP to protect the neurotoxicity in hydro peroxide-treated HMO6 cells. Taken together, we demonstrated that ECAP may inhibit hydro peroxide-mediated neurotoxicity through the regulation of p53 and Bcl-2 genes in HMO6 cells and ameliorate oxidative stress in hydro peroxide-treated HMO6 cells. Therefore we suggest that ECAP may hold the potential to prevent the incidence of neuronal damage-mediated neurodegenerative diseases.},
doi={10.12691/jfnr-5-3-8}
publisher={Science and Education Publishing}
}