eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2017-03-11 5 3 176 179 10.12691/jfnr-5-3-6 JFNR2017536 article Myo Jin Kwon 1 Kui-Jin Kim 2 Byung Wook Yang 3 Boo-Yong Lee 3 Hyoung Chun Kim kimhc@kangwon.ac.kr; skko@semyung.ac.kr 4 Sung Kwon Ko kimhc@kangwon.ac.kr; skko@semyung.ac.kr 4 4 Department of Oriental Medical Food & Nutrition, Semyung University, Jecheon, Republic of Korea Department of Food Science and Biotechnology, CHA University, Seongnam, Republic of Korea Ginseng Research Center, Koyeon, Jecheon, Republic of Korea College of Pharmacy, Kangwon National University, Chunchon, Republic of Korea Ginseng is known to contain numerous ginsenosides, which exhibit beneficial health effects. In particular, ginsenoside Rg3, Rg5, and Rk1, derived from high temperature-processed ginseng, have higher bioactivities compared to other types of ginsenosides. In this study, we determined the optimal conditions for extraction of the valuable ginsenosides Rg3, Rg5, and Rk1 from black ginseng. Our results revealed that black ginseng extracted with 100°C distilled water for 0.5 hours and 6 hours yields large amounts of Rg3, Rg5, and Rk1 compared with other extraction times. We also found that protopanaxadiol (PPD) and protopanaxatriol (PPT) were also efficiently extracted from black ginseng with 100 °C distilled water at 0.5 hours and 6 hours and then rapidly degraded in a time-dependent manner. Therefore, we posit that short extraction times such as 0.5 hours and 6 hours might be optimal for isolation of the ginsenosides Rg3, Rg5, and Rk1 from black ginseng as opposed to longer extraction periods. http://pubs.sciepub.com/jfnr/5/3/6/jfnr-5-3-6.pdf black ginseng ginsenoside prosapogenin protopanaxadiol protopanaxatriol Rg3 Rg5 Rk1