Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 4 9 2016 9 9 Low Cytotoxic D-mannitol Isolated from the Industrial Wastewater of Agaricus bisporus 610 614 EN Jinmei Lin Jiafu Huang Junyu Chen Zhichao Lin Yixin Ou Liyun Yao Xiufen Zhang Qianjin Kang Yutian Pan Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, China JFNR2016498 10.12691/jfnr-4-9-8 2016 4 20 2016 7 12 2016 9 7 Agaricus bisporus is an edible mushroom, and mainly made in canned food for storage and trading. However, large amount of industrial wastewater containing various components is generated during the production of canned mushroom. This study describes the method for purification of D-mannitol and evaluation of its bioactivity from the industrial wastewater of A. bisporus. Its structure was confirmed by GC, IR, UV, HPLC, HPLC-MS, DSC analysis. The content of D-mannitol in the concentrated industrial wastewater (≥45°Be') of A. bisporus was determined to more than 32.0%. Comparing with the D-mannitol standard, this purified MN6 had a higher purity and lower cytotoxicity to L929 fibroblast evaluated by MTS assay. These results revealed that the A. bisporus industrial wastewater is a good resource for obtanning natural D-mannitol with high quality.