@article{jfnr2016498,
author={{Lin, Jinmei and Huang, Jiafu and Chen, Junyu and Lin, Zhichao and Ou, Yixin and Yao, Liyun and Zhang, Xiufen and Kang, Qianjin and Pan, Yutian},
title={Low Cytotoxic D-mannitol Isolated from the Industrial Wastewater of <i>Agaricus bisporus</i>},
journal={Journal of Food and Nutrition Research},
volume={4},
number={9},
pages={610--614},
year={2016},
url={http://pubs.sciepub.com/jfnr/4/9/8},
issn={2333-1240},
abstract={<i>Agaricus bisporus</i><i> </i>is an edible mushroom, and mainly made in canned food for storage and trading. However, large amount of industrial wastewater containing various components is generated during the production of canned mushroom. This study describes the method for purification of D-mannitol and evaluation of its bioactivity from the industrial wastewater of <i>A</i><i>.</i><i> bisporus</i>. Its structure was confirmed by GC, IR, UV, HPLC, HPLC-MS, DSC analysis. The content of D-mannitol in the concentrated industrial wastewater (¡Ý45¡ãBe') of <i>A</i><i>.</i><i> bisporus</i><i> </i>was determined to more than 32.0%. Comparing with the D-mannitol standard, this purified MN6 had a higher purity and lower cytotoxicity to L929 fibroblast evaluated by MTS assay. These results revealed that the <i>A. bisporus</i> industrial wastewater is a good resource for obtanning natural D-mannitol with high quality.},
doi={10.12691/jfnr-4-9-8}
publisher={Science and Education Publishing}
}