@article{jfnr2016497,
author={{Kao, Ning-Jo and Wu, Chien-Sheng and Hu, Jia-Yuan and Kong, Zwe-Ling},
title={<i>S</i>-nitrosylation of Inhibitor-¦ÊB kinase: Identifying Novel Targets of Curcumin},
journal={Journal of Food and Nutrition Research},
volume={4},
number={9},
pages={600--609},
year={2016},
url={http://pubs.sciepub.com/jfnr/4/9/7},
issn={2333-1240},
abstract={In this study, we investigated the preventive effects of curcumin using LPS (Lipopolysaccharides)-induced Raw264.7 cells and the potential role of curcumin in regulation of anti-inflammation through <i>S</i>-nitrosylation. Western blot presented the protein expression of iNOS can be reduced by treated curcumin with 5, 10 and 15 ¦ÌM separately for 12 and 24h. Consistently, pro-inflammatory cytokines, such as IL-1¦Â, IL-6, TNF¦Á and IFN-¦Ã was also repressed. Moreover, 5, 10 and 15 ¦ÌM curcumin reduced the amount of nitrite and nitrate in LPS-induced Raw264.7 cells maintained total <i>S</i>-nitrosylation level on proteins at 12h, the similar results was also observed at 24h that indicating curcumin inhibited NO oxidation. Furthermore, the protection of <i>S</i>-nitrosylation on IKK¦Â in LPS-induced Raw264.7 cells at 12h by curcumin caused the repression of I¦ÊB phosphorylation and NF-¦ÊB activation. In conclusion, this study verified that curcumin-mediated <i>S</i>-nitrosylation may be as an important regulator for anti-inflammation in LPS-induced Raw264.7 cells.},
doi={10.12691/jfnr-4-9-7}
publisher={Science and Education Publishing}
}