@article{jfnr2016483,
author={{Ma, Yi-Shing and Yoshida, Shigeru and Kobayashi, Yu and Kawanishi, Noriaki and Furukawa, Takayuki and Fuda, Hirotoshi and Hui, Shu-Ping and Chiba, Hitoshi},
title={Improvement of Mitochondrial Function and Lipid Utilization by 3,5-dihydroxy-4-methoxybenzyl Alcohol, an Oyster-derived polyphenol, in Oleate-loaded C2C12 Myotubes},
journal={Journal of Food and Nutrition Research},
volume={4},
number={8},
pages={498--507},
year={2016},
url={http://pubs.sciepub.com/jfnr/4/8/3},
issn={2333-1240},
abstract={Anti-oxidative effects of the Pacific oyster-derived phenolic antioxidant, 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), has been documented in hepatocytes. Additionally, DHMBA-rich oyster extracts significantly attenuated obesity in a non-alcoholic steatohepatitis mouse model. Whether the administration of DHMBA might improve muscular mitochondrial function was investigated. The mouse C2C12-derived myotubes were loaded with oleic acid (400&#956;M) and cultured for 24 hours in the presence of DHMBA (500&#956;M) with or without electrical stimulation (ES), where ES was given as exercise mimic. The fatty acid uptake, lipid accumulation, and mitochondrial function were subsequently accessed.<b> </b>DHMBA and ES increased fatty acid uptake, TG contents, mitochondrial membrane potential, intracellular level of H<SUB>2</SUB>O<SUB>2</SUB>, and mitochondrial O<SUB>2</SUB> consumption rate. Intracellular ATP content was significantly increased when both DHMBA and ES were loaded at the same time, suggesting their synergic action. Phosphorylated AMPK¦Á, AMPK¦Â1, and acetyl-CoA carboxylase were increased by DHMBA, indicating a possible role for DHMBA for activation of metabolic adaptation system and consequent increase of fatty acid oxidation. In conclusion, DHMBA solely or in collaboration with exercise might possibly serve as a fitness food for obese persons by stimulating muscular fatty acid utilization and mitochondrial energy production. This assumption must be verified by animal experiment.},
doi={10.12691/jfnr-4-8-3}
publisher={Science and Education Publishing}
}