Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 4 6 2016 7 5 Anti-obesity Effect and Antioxidant Activity in High-fat Diet Mice Fed Fermented Buckwheat Products (Miso) 355 360 EN Shan Wu Shuo Feng ZhaoHong Ci Chengyu Jiang Yang Cui Yuki Sasaki Yukina Ota Michiyuki Kojima Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine, 11, Nishi-2-sen, Inada-machi, Obihiro, Hokkaido 080-8555, Japan JFNR2016463 10.12691/jfnr-4-6-3 2016 2 15 2016 5 5 2016 7 3 Here we examined the effects of consuming fermented buckwheat products in mice fed a high-fat diet. Buckwheat was fermented with soybeans (1:1) and rice koji for 6 months (miso). The total phenolic content and 1,1-diphenyl-2-picrylhydrazyl content of the buckwheat fermentation products (miso) increased with the time of fermentation. In the in vivo experiments, mice were fed for 5 weeks with a high-fat diet with or without supplementation using 10% buckwheat miso. Dietary supplementation with buckwheat miso significantly decreased the serum aspartate transaminase level and lipid peroxidation in the liver compared with the control group. Furthermore, the mice that received buckwheat miso showed significant decreases in their body weight and retroperitoneal and mesenteric fat weights and increased fecal lipid contents compared with the control group. These results indicate that dietary supplementation with buckwheat, which is characterized by a high antioxidant activity, has health benefits such as protection of the liver and suppression of increased body weight.