eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2016-03-21 4 2 126 130 10.12691/jfnr-4-2-10 JFNR20164210 article Yeon-Joo Lee 1 Kye-Yoon Yoon 1 Ok-Hwan Lee 2 Kui-Jin Kim Kuijin.Kim@gmail.com, bylee@cha.ac.kr 2 Boo-Yong Lee Kuijin.Kim@gmail.com, bylee@cha.ac.kr 2 Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam, Kyonggi, South Korea Department of Food Science and Biotechnology, Kangwon National University, Chuncheon, South, Korea Previous study suggested that polyphenol-enriched extracts from Ecklonia cava (PREC, Seapolynol) have an inhibitory effect on lipid accumulation in vitro and in vivo. Based on these results, we evaluated the effect of PREC on insulin-mediated glucose uptake in 3T3-L1 cells and in male diabetic C57BL/KsJ-db/db mice. The mice were divided into four groups, including db/db, Rosiglitazone 10mg/kg/day, PREC 60mg/kg/day and PREC 150mg/kg/day. Treatment with PREC upregulated glucose uptake-associated gene expression and improved glucose uptake in fully differentiated 3T3-L1 adipocytes. Additionally, α-glucosidase (α-carbohydrate-hydrolase) was inhibited by PREC in a dose-dependent manner. Moreover, PREC effectively improved GLUT4-associated gene expression and suppressed fasting blood glucose levels. These results suggest that PREC may have a mitigating effect on hyperglycaemia and could help to improve blood glucose levels in diabetes patients. http://pubs.sciepub.com/jfnr/4/2/10/jfnr-4-2-10.pdf 3T3-L1 Seapolynol diabetes insulin sensitivity