Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 4 10 2016 10 15 Inhibitory Effects and Molecular Mechanism of an Anti-inflammatory Peptide Isolated from Intestine of Abalone, Haliotis Discus Hannai on LPS-Induced Cytokine Production via the p-p38/p-JNK Pathways in RAW264.7 Macrophages 690 698 EN Zhong-Ji Qian BoMi Ryu Won Sun Park IL-Whan Choi Department of Microbiology, College of Medicine, Inje University, Busan 633-165, Republic of Korea Won-Kyo Jung Department of Biomedical Engineering, and Center for Marine-Intergrated Biomedical Technology (BK21 Plus) Pukyong National University, Busan 608-737, Republic of Korea JFNR20164109 10.12691/jfnr-4-10-9 2016 7 11 2016 7 27 2016 10 13 A marine mollusk, abalone (Haliotis discus hannai) is one of the important species in aquaculture industry, but nutraceutical and pharmaceutical benefits of H. discus hannai have been rarely identified and studied. To evaluate beneficial effects of H. discus hannai, an anti-inflammatory peptide (AAIP, abalone anti-inflammatory peptide) was purified from abalone intestines using consecutive HPLC purification system. In tandem MS analysis, the fragmentation results illustrate that the AAIP responsible for the nitric oxide (NO) inhibitory activity (IC50=55.8μM) has amino acid sequence as Pro-Phe-Asn-Glu-Gly-Thr-Phe-Ala-Ser (1175.2 Da). To investigate anti-inflammatory effect of AAIP on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and elucidated the molecular mechanism. The results show that the AAIP peptide suppresses LPS-induced production of nitric oxide (NO) via inducible nitric oxide synthase (iNOS) expression in a dose-dependent manner. It also significantly reduced the gene transcription of proinflammatory cytokines, such as interleukin-1̦ (IL-1β), IL-6, and tumor necrosis factor-β (TNF-β). Furthermore, AAIP significantly suppresses phosphorylation of mitogen-activated protein kinases (MAPKs) such as p-p38 and p-JNK. These results indicated that AAIP inhibits LPS-induced inflammatory response via blocking of MAPK pathway in murine macrophages. Therefore, potent AAIP might suggest possibility for high valuable utilization and application as nutraceutical and therapeutic substances.