@article{jfnr2015393,
author={{Yoon, Kye-Yoon and Kim, Kui-Jin and Youn, Hyung-Sun and Oh, Sei-Ryang and Lee, Boo-Yong},
title={Brazilin Suppresses Inflammation via the Down-regulation of IRAK4 in LPS-stimulated Raw264.7 Macrophage},
journal={Journal of Food and Nutrition Research},
volume={3},
number={9},
pages={575--580},
year={2015},
url={http://pubs.sciepub.com/jfnr/3/9/3},
issn={2333-1240},
abstract={Brazilin, is a bioactive compound extracted from <i>Caesalpinia sappan Linn</i>, has been reported the protective effect of the immune system. Particular attention is now devoted to better understanding of the molecular basis of bazilin anti-inflammatory activity. In the present study, we studied the effect of brazilin on the Raw264.7 macrophage cell lines by a nutrigenomics approaches. Raw264.7 cells were treated with braziln, then treated with LPS to cause inflammation. The nuclear transcription ¦ÊB (NF-¦ÊB) promoter activity were analyzed with dual luciferase assay kit. The gene expression and production levels of pro-inflammatory cytokine interleukin (IL)-1¦Â, tumor necrosis factor (TNF)¦Á, and IL-6 were evaluated with semi-quantitative RT-PCR and with ELISA, respectively. We also examined inflammatory signaling, including mitogen-activated protein kinase (MAPK) pathway, iNOS, COX2, and IRAK4. Our findings demonstrated that brazilin down-regulated the expression of IRAK4 protein lead to suppress of c-Jun NH<SUB>2</SUB> terminal kinase (JNK) signaling, and subsequently inactivation of nuclear transcription ¦ÊB (NF-¦ÊB), inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) thus promoting the expression of the downstream target pro-inflammatory cytokines such as IL-1¦Â, TNF¦Á and IL-6 in LPS stimulated Raw264.7 macrophage cell. Thus, brazilin showed anti-inflammatory activity in Raw264.7 macrophage cell targeting IRAK4 mediated signaling pathway.},
doi={10.12691/jfnr-3-9-3}
publisher={Science and Education Publishing}
}