@article{jfnr2015388,
author={{Chen, Tai-Yuan and Chang, Yu-Huai and Lin, Hsi-Pin and Chen, Shui-Tein and Hwang, Deng-Fwu},
title={Proteomic Identification of Stonefish <i>Synanceja verrucosa</i> Venom},
journal={Journal of Food and Nutrition Research},
volume={3},
number={8},
pages={526--539},
year={2015},
url={http://pubs.sciepub.com/jfnr/3/8/8},
issn={2333-1240},
abstract={Three venom toxins, neoverrucotoxin (neoVTX) ¦Á-subunit and ¦Â-subunit as well as verrucotoxin (VTX) ¦Â-subunit, were identified in the stonefish <i>Synanceja verrucosa</i> by SDS-PAGE, Native-PAGE and two-dimensional electrophoresis (2-DE) coupled with Matrix Assisted Laser Desorption Ionization-Quadrupole-Time-of-Flight (MALDI-Q-TOF). The venom estimated by Native-PAGE were 471, 358, 260 and 166 kDa. The predominate protein bands of crude venom were 84 and 75 kDa by SDS-PAGE. The crude venom protein fell in the region with p<i>I</i> values of 7-9 and molecular weights of 75-90 kDa by 2-DE. Peptide mass fingerprints (PMF) and MS/MS ions originated from MALDI-Q-TOF were used to identify the protein. Our results showed that the complete components of neoverrucotoxin (neoVTX) ¦Á-subunit and ¦Â-subunit as well as verrucotoxin (VTX) ?¦Â-subunit were identified from SDS-PAGE and 2-DE patterns. Native-PAGE did not yield protein identifications but revealed the presence of protein complexes.},
doi={10.12691/jfnr-3-8-8}
publisher={Science and Education Publishing}
}