Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 3 7 2015 09 09 Bioactivity of -1,3-xylan Extracted from Caulerpa lentillifera by Using Escherichia coli ClearColi BL21(DE3)--1,3-xylanase XYLII 437 444 EN Wen-Sing Liang Tristan C. Liu Chun-Ju Chang Chorng-Liang Pan Department of Food Science, National Taiwan Ocean University, Jhongjheng District, Keelung City, Taiwan JFNR2015375 10.12691/jfnr-3-7-5 2015 08 17 2015 08 31 2015 09 09 Oligosaccharides extracted from algae exhibit many bioactivities and are used as food additives and dietary supplements. In this study, -1,3-xylan was extracted from the green algae Caulerpa lentillifera; this compound was hydrolyzed by -1,3-xylanase XYLII to produce mixed < 3 kDa -1,3-xylooligosaccharide (XOSmix), which was mainly composed of -1,3-xylose, -1,3-xylobiose, and -1,3-xylotriose. The antioxidant and anticoagulant activities of XOSmix were then examined. Results revealed that the 2,2-diphenyl-1-pikryl-hydrazyl scavenging activity, reducing power, and total antioxidant status of 20 mg/mL XOSmix was equivalent to those of 8.7, 115.1, and 157.3 μg/mL trolox, respectively; whereas the ferrous ion chelating activity of 20 mg/mL XOSmix was equivalent to that of 64.3 g/mL EDTA. Regarding the anticoagulant activity, XOSmix delayed the activated partial thromboplastin time. These results suggest that XOSmix exhibits potential for application in the food industry.