Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 3 6 2015 07 09 Reproductive Effects of 3-Monochloropropane-1, 2-diol on Mice Sperm Function and Early Embryonic Development In Vitro 405 409 EN Luona Wen Jianxia Sun Shun Bai Yunfeng Hu Shi Wu Rui Jiao Shiyi Ou Weibin Bai JFNR2015368 10.12691/jfnr-3-6-8 2015 06 04 2015 06 22 2015 07 09 3-Monochloropropane-1, 2-diol (3-MCPD) is a well-known chloropropanol that is synthesized during food processing contamination. While evaluating sperm quality, sperm penetration into oocytes and early embryonic development, the present study investigated the effects of 3-MCPD onmicereproduction in vitro. During thein vitro fertilization (IVF) process, zygotes and 2-cell embryos were incubated with 3-MCPD-supplemented medium until 8-cell embryo formation to evaluate the reproductive toxicity of 3-MCPD. Our data showed that compared with the control group, the fertilization rate and cleavage rate remarkably decreased in groups treated with 1.802 and 3.160 mM 3-MCPD. The percentage of 2-cell embryo and 4-cell embryo formation also significantly decreased (P < 0.05). Moreover, 8-cell embryos were not observed in any of the treated groups. Taken together, these results suggest that 3-MCPD exposure has hazardous effects on the micesperm fertilization ability as well as embryonic development.