eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2015-07-09 3 6 405 409 10.12691/jfnr-3-6-8 JFNR2015368 article Luona Wen 1 Jianxia Sun 2 Shun Bai 2 Yunfeng Hu 2 Shi Wu 2 Rui Jiao 2 Shiyi Ou 2 Weibin Bai 2 Department of Food Science and Engineering, Department of Developmental and Regenerative Biology, Biopharmaceutical R&D Center, Jinan University, Guangzhou, China Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, China 3-Monochloropropane-1, 2-diol (3-MCPD) is a well-known chloropropanol that is synthesized during food processing contamination. While evaluating sperm quality, sperm penetration into oocytes and early embryonic development, the present study investigated the effects of 3-MCPD onmicereproduction in vitro. During thein vitro fertilization (IVF) process, zygotes and 2-cell embryos were incubated with 3-MCPD-supplemented medium until 8-cell embryo formation to evaluate the reproductive toxicity of 3-MCPD. Our data showed that compared with the control group, the fertilization rate and cleavage rate remarkably decreased in groups treated with 1.802 and 3.160 mM 3-MCPD. The percentage of 2-cell embryo and 4-cell embryo formation also significantly decreased (P < 0.05). Moreover, 8-cell embryos were not observed in any of the treated groups. Taken together, these results suggest that 3-MCPD exposure has hazardous effects on the micesperm fertilization ability as well as embryonic development. http://pubs.sciepub.com/jfnr/3/6/8/jfnr-3-6-8.pdf 3-Monochloropropane-1 2-diol; mouse sperm function; early embryonic development