Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 3 5 2015 05 17 Immunomodulation Effect of Metabolites from Lactobacillus Rhamnosus GG on Interleukins Release in Vitro 297 302 EN Edyta Balejko Department of Human Nutrition, West Pomeranian University of Technology in Szczecin, Poland Anna Bogacka Jerzy Balejko Elżbieta Kucharska JFNR2015352 10.12691/jfnr-3-5-2 2015 03 18 2015 05 06 2015 05 17 The aim of the study was to determine in vitro whether the metabolites of Lactobacillus rhamnosus GG stimulate the release of selected cytokines from lymphocytes. Cell cultures from whole human blood were established. The concentration of TGF-β1, IFN-γ and IL-4 was determined in cultures liquid by ELISA. Lactobacillus GG rods were immobilized in alginate microcapsules. To determine the tightness of the capsules their surfaces were observed by electron microscopy. During 14 days the migration of the bacteria to outside of the capsules was not observed. In liquid from cell cultures of lymphocytes the increase of TGF-æ1 and IL-4 and the decrease in IFN-¦ concentration were observed, influenced by Lactobacillus GG metabolites enclosed in lyophilized alginate microcapsules, as compared to control group. The possible stimulants were exopolysaccharide and Lactobacillus GG metabolites, i.e. lactic acid, nitrogen oxide and hydrogen peroxide.