eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2015-05-17 3 5 297 302 10.12691/jfnr-3-5-2 JFNR2015352 article Edyta Balejko Edyta.Balejko@zut.edu.pl 1 Anna Bogacka 1 Jerzy Balejko 2 Elżbieta Kucharska 2 Department of Human Nutrition, West Pomeranian University of Technology in Szczecin, Poland Department of Food Process Engineering, West Pomeranian University of Technology in Szczecin, Poland The aim of the study was to determine in vitro whether the metabolites of Lactobacillus rhamnosus GG stimulate the release of selected cytokines from lymphocytes. Cell cultures from whole human blood were established. The concentration of TGF-β1, IFN-γ and IL-4 was determined in cultures liquid by ELISA. Lactobacillus GG rods were immobilized in alginate microcapsules. To determine the tightness of the capsules their surfaces were observed by electron microscopy. During 14 days the migration of the bacteria to outside of the capsules was not observed. In liquid from cell cultures of lymphocytes the increase of TGF-æ1 and IL-4 and the decrease in IFN-¦ concentration were observed, influenced by Lactobacillus GG metabolites enclosed in lyophilized alginate microcapsules, as compared to control group. The possible stimulants were exopolysaccharide and Lactobacillus GG metabolites, i.e. lactic acid, nitrogen oxide and hydrogen peroxide. http://pubs.sciepub.com/jfnr/3/5/2/jfnr-3-5-2.pdf microencapsulation immunomodulation TGF-æ1 IFN-¦ IL-4 probiotics