@article{jfnr2015352,
author={{Balejko, Edyta and Bogacka, Anna and Balejko, Jerzy and Kucharska, El?bieta},
title={Immunomodulation Effect of Metabolites from <i>Lactobacillus Rhamnosus GG</i> on Interleukins Release <i>in Vitro</i>},
journal={Journal of Food and Nutrition Research},
volume={3},
number={5},
pages={297--302},
year={2015},
url={http://pubs.sciepub.com/jfnr/3/5/2},
issn={2333-1240},
abstract={The aim of the study was to determine <i>in vitro</i> whether the metabolites of <i>Lactobacillus rhamnosus GG</i> stimulate the release of selected cytokines from lymphocytes. Cell cultures from whole human blood were established. The concentration of TGF-¦Â1, IFN-¦Ã and IL-4 was determined in cultures liquid by ELISA. <i>Lactobacillus GG</i> rods were immobilized in alginate microcapsules. To determine the tightness of the capsules their surfaces were observed by electron microscopy. During 14 days the migration of the bacteria to outside of the capsules was not observed. In liquid from cell cultures of lymphocytes the increase of TGF-¦Â1 and IL-4 and the decrease in IFN-¦Ã concentration were observed, influenced by <i>Lactobacillus GG</i> metabolites enclosed in lyophilized alginate microcapsules, as compared to control group. The possible stimulants were exopolysaccharide and <i>Lactobacillus GG</i> metabolites, i.e. lactic acid, nitrogen oxide and hydrogen peroxide.},
doi={10.12691/jfnr-3-5-2}
publisher={Science and Education Publishing}
}