@article{jfnr2015313,
author={{Zhang, Xifeng and Zhang, Fenqin and Luo, Guanghong and Yang, Shenghui and Wang, Danxia},
title={Extraction and Separation of Phycocyanin from <i>Spirulina</i> using Aqueous Two-Phase Systems of Ionic Liquid and Salt},
journal={Journal of Food and Nutrition Research},
volume={3},
number={1},
pages={15--19},
year={2015},
url={http://pubs.sciepub.com/jfnr/3/1/3},
issn={2333-1240},
abstract={To explore a new and simple rapid extraction and purification technique for phycocyanin, an ionic liquids(ILs)-based aqueous two-phase system(ATPS) was developed for the purification of phycocyanin from <i>Spirulina</i> extracts. Effects of various process parameters such as the concentrations of [Bmim]Cl, the concentrations of KH<SUB>2</SUB>PO<SUB>4</SUB>, the concentrations of crude phycocyanin, the system pH and the temperature on partitioning of phycocyanin were evaluated. The obtained data indicated that phycocyanin was preferentially partitioned into the ILs-rich phase and the ATPS composed of 23% (w/w) [Bmim]Cland 29% (w/w) KH<SUB>2</SUB>PO<SUB>4</SUB> at 30ˇăC and pH 7.0 showed good selectivity on phycocyanin. Under the optimum conditions, phycocyanin with a purity of 3.98 and yield of about 90.23 % was obtained. Therefore, ILs-based ATPS was an effective method for partitioning and recovery of phycocyanin from <i>Spirulina</i> extracts.},
doi={10.12691/jfnr-3-1-3}
publisher={Science and Education Publishing}
}