eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2014-08-14 2 8 506 509 10.12691/jfnr-2-8-12 JFNR20142812 article Mian Anjum Murtaza anjum_ft@yahoo.com 1 Nuzhat Huma 2 Zafar Hayat 3 Mian Shamas Murtaza 3 Asif Meraj 3 Institute of Food Science and Nutrition, University of Sargodha, Sargodha, Pakistan National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan Department of Animal Sciences, University of Sargodha, Sargodha, Pakistan The aim of the study was to evaluate the effect of feed management on conjugated linoleic acid (CLA) level in milk and subsequently on fatty acid profile of cheese. Twenty cows were divided into 2 groups, fed on grass silage and pasture feed with sunflower supplementation for 14 days. Milk samples were collected and analyzed for composition and fatty acid profile using gas chromatograph. Cheddar cheese was manufactured from both milk types and ripened for 120 days. During ripening, it was analyzed for composition and fatty acid quantification. The supplemented diet significantly increased the CLA content in milk. Similarly the cis-9, trans-11 CLA content in Cheddar cheese were more than twofold in case of supplemented diet as compared to grass silage. During ripening, CLA concentration remained stable. The sunflower supplementation showed no effect on cheese composition. Hence, supplemented pasture resulted in elevated CLA content in milk compared to grass silage. http://pubs.sciepub.com/jfnr/2/8/12/jfnr-2-8-12.pdf milk fatty acids cheddar cheese conjugated linoleic acid