Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2 7 2014 07 21 The Comparison and Analysis of Three Extraction Methods for Polysaccharides in Purslane 401 405 EN Chunmei GU Institute of Food Science and Engineering, Jilin Agricultural University, Changchun, China Shu PAN JFNR20142712 10.12691/jfnr-2-7-12 2014 06 27 2014 07 17 2014 07 21 In this study, three extraction methods of polysaccharides in Purslane were compared and analyzed, and the best method was ascertained. Purslane polysaccharides were extracted using water, ultrasonic and microwave. By the results of single factor experiments and orthogonal experiments, the optimal extracting conditions were selected and then the best extraction method was determined. In order to calculate the extraction yield of polysaccharides, its content were measured by the pheno-sulfuric acid method using glucose as the standard, and then the efficiency of three extraction methods was evaluated. The results showed that the extraction yield of ultrasonic was 35.42% under the optimal conditions of 60C, 60 min, 1:20 and 200w. The extraction yield of microwave method was 32.12% under the optimal conditions of 3 min, 1:25 and 500w. The extraction yield of water method was 31.22% under the optimal conditions of 80C, 2 h, 1:30 and 3 times. It could be concluded that ultrasonic method was the best one in these three methods.