@article{jfnr20142410,
author={{Eibaid, Ahmed and Bashari, Mohanad and Miao, Ming and Musa, Abubakr and Zhang, Tao and Jiang, Bo},
title={Biosynthesis of 2-O-¦Á-D-glucopyranosyl-L-Ascorbic Acid from Maltose by Cyclodextrin Glucanotransferase from Bacillus sp. SK 13.002},
journal={Journal of Food and Nutrition Research},
volume={2},
number={4},
pages={193--197},
year={2014},
url={http://pubs.sciepub.com/jfnr/2/4/10},
issn={2333-1240},
abstract={In this work 2-O-¦Á-D-glucopyranosyl-L-ascorbic acid (AA-2G) was synthesized by Cyclodextrin glucanotransferase (CGTase) from <i>Bacillus </i>sp. SK 13.002 with L-ascorbic acid (AA) as an acceptor and maltose as a glycosyl donor. AA-2G production was analyzed by HPLC and was confirmed by LC/MS results. The reaction parameters, such as pH (4.0-9.0), temperature (25-50C), time (0-30 h), substrate ratios and enzyme concentration were optimized. The results showed that the optimum condition was pH 8.0 at 37C for 24 h, 1:1 maltose to AA substrates mass ratio, and 200 U/mL of CGTase. Under these conditions, the production of AA-2G was 5.5 g/L, this result indicate that CGTase from Bacillus sp. SK 13.002 can effectively uses maltose as a glycosyl donor to produce AA-2G in high yield.},
doi={10.12691/jfnr-2-4-10}
publisher={Science and Education Publishing}
}