Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2 3 2014 04 09 The Immunostimulatory and Anti-tumor Activities of Polysaccharide from Agaricus bisporus (brown) 122 126 EN Yanqing Zhang Guijie Ma Leilei Fang Lijuan Wang Junbo Xie College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China JFNR2014235 10.12691/jfnr-2-3-5 2014 03 11 2014 03 31 2014 04 09 Agaricus bisporus is one of the most consumed culinary mushrooms in the world. The white type of Agaricus bisporus has been demonstrated with various bioactivities beneficial to human health. However, little was known about its brown type. In this study, the anti-cancer and immunoregulatory potential of Agaricus bisporus (brown) polysaccharide were investigated in vitro and in vivo. In vitro, the polysaccharide had a significant antiproliferative effect on Hela cells (cervical cancer cells), especially at the high concentration of 200 µg/ml. The spleen index, thymus index and carbon clearance ability of mice were remarkably increased by the polysaccharide treatment, indicating the polysaccharide had a promising benefit for immunodulation in vivo. Furthermore, the polysaccharide increased the proliferation of RAW264.7 cells with 19.1% at 50µg /ml and 34.5% at 100µg /ml, which demonstrated the immunodulation mechanism was in close conjunction with macrophages. In conclusion, these results indicated that Agaricus bisporus (brown) polysaccharide possessed strong immunostimulatory and anti-tumor bioactivity in vivo and in vitro. Therefore, it could be explored as a novel natural antitumor agent with immunomodulatory activity.