@article{jfnr201421219,
author={{Hong, Housheng and Min, Zhaosheng and Guo, Huiming},
title={Control of the Ratio of Inducer to Cell Concentration to Enhance the Phytase Production in Recombinant <i>Pichiapastoris</i>},
journal={Journal of Food and Nutrition Research},
volume={2},
number={12},
pages={980--984},
year={2014},
url={http://pubs.sciepub.com/jfnr/2/12/19},
issn={2333-1240},
abstract={Phytase production by <i>Pichiapastoris</i> was used as a case to study the mechanism and strategy for optimization of heterologous protein production. It was found that the ratio of inducer-methanol to cell concentration had a significant influence on phytase production. In this case, we found that the optimum initial cell concentration and methanol concentration were 85 g/L and 10 g/L, respectively. During induction period, an easy-to-control methanol feeding method was proposed according to the optimal ratio of methanol to cell concentration at a range of 0.063 -0.132 g/g, and phytase activity and productivity reached 53984 U/mL and 529.25 U/(mL¡¤h), respectively. The method for optimization of phytase production through controlling the optimal ratio maybe provide an alternative idea to enhance other heterologous protein production with <i>P. pastoris</i>.},
doi={10.12691/jfnr-2-12-19}
publisher={Science and Education Publishing}
}