eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2014-10-26 2 11 814 818 10.12691/jfnr-2-11-9 JFNR20142119 article Fa-yi Hao 1 Li-xin Lu lulx@jiangnan.edu.cn 1 2 Chang-feng Ge 3 Jiangnan University, Wuxi, P.R. China Rochester Institute of Technology, 78 Lomb Memorial Drive, Rochester, NY 14623, USA Moisture sorption isotherm is a well established method to characterize water sorption properties and behavior of food materials. However, this approach doesn't adequately reflect the molecular mobility that taking place during water sorption process. Nuclear magnetic resonance (NMR) can provide information about the water mobility and molecular interactions between water and food components. The biscuits with different fat addition were studied using water sorption isotherm and 1H low-field NMR at and water activity ranging from 0.2 to 0.90, the changes in equilibrium moisture content, transverse relaxation time(T2) and proton intensity of biscuits were defined. The T2 were measured with Carr-Purcell-Meiboom-Gill (CPMG) sequences. It was demonstrated that fat content of biscuits influenced directly the equilibrium moisture content and water status. One or two water populations were observed as the water activity increased, each of which had a distinct relaxation time T2 or molecular mobility. The relaxation time manifested that with the increase of fat addition, the water inside the samples became more mobile, and proton intensity indicated that the amount of water uptake decreased with increasing fat addition. The low-field NMR was demonstrated to provide complementary interpretation to that of water sorption isotherm. http://pubs.sciepub.com/jfnr/2/11/9/jfnr-2-11-9.pdf Nuclear magnetic resonance water sorption isotherm transverse relaxation time biscuit fat content