Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2 11 2014 10 26 Lifetime Assessment of Dietary Nucleotides Consumption in Sprague-Dawley Rats 806 813 EN Meihong Xu Rui Liang Qianying Guo Shuangjia Wang Ming Zhao Zhaofeng Zhang Junbo Wang Yong Li Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, PR China JFNR20142118 10.12691/jfnr-2-11-8 2014 04 29 2014 10 24 2014 10 26 To assess the long-term toxicity and carcinogenicity of dietary nucleotides (NTs), Sprague-Dawley rats were administered the NTs for life-through with the dose of 0, 0.01%, 0.04%, 0.16% and 0.64%. With the exception of several groups, the results showed no significant differences in weight gain, clinical symptoms data, blood indicators, and Non-neoplastic lesions frequencies. NTs have dramatically increased the food consumption of both sexes (P<0.05). Meanwhile, NTs decreased spontaneous tumor incidence of both sexes, notably the male rats (P<0.05). The occurrence of all malignant and mammary tumors in NTs-treated females was lower than those in controls (P<0.05). In comparison with the control group, the incidence of death from malignant and systemic tumors were obviously decreased in the NTs-treated groups (P<0.01). Moreover, our results showed that a higher percentage of rats in the NTs-treated groups died of non-neoplastic lesions compared with control. In conclusion, dietary nucleotides are not toxic or carcinogenic in rats up to 0.64% for all the life, and 0.64% was an unobservable e?ect dose.