eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2023-09-22 11 9 594 601 10.12691/jfnr-11-9-6 JFNR20231196 article Alfonso Topete-Betancourt 1 Luis E. S¨¢mano Mu?oz 1 H¨¦ctor E. Mart¨ªnez-Flores hector.martinez.floresauthor@umich.mx 1 Osvaldo ¨¢lvarez-Cort¨¦s 2 Jose J. Virgen Ortiz 3 M.G. Garnica-Romo 4 Luis F. Ortega-Varela 5 Rosa M. Garc¨ªa-Mart¨ªnez 5 Facultad de Qu¨ªmico Farmacobiolog¨ªa. Universidad Michoacana de San Nicolas de Hidalgo, Tzintzuntzan 173, Col. Matamoros. C.P. 58240. Morelia, Michoac¨¢n, M¨¦xico, Programa Institucional de Doctorado en Ciencias Biol¨®gicas. Universidad Michoacana de San Nicol¨¢s de Hidalgo. Avenida Francisco J. M¨²gica. S/N. Ciudad Universitaria. C.P. 58030. Morelia, Mich., M¨¦xico. CONAHCYT - Centro de Investigaci¨®n en Alimentaci¨®n y Desarrollo, A. C. ¨C CIDAM, Antigua Carretera a P¨¢tzcuaro km. 8. C.P. 58341. Morelia, Michoac¨¢n, M¨¦xico. Facultad de Ingenier¨ªa Civil. Universidad Michoacana de San Nicol¨¢s de Hidalgo. Santiago Tapia 403. Col. Centro. C.P. 58000. Morelia, Mich., M¨¦xico. Facultad de Salud P¨²blica y Enfermer¨ªa. Gertrudis Bocanegra 330, Cuauht¨¦moc. C.P. 58000. Morelia, Mich., M¨¦xico. Cannabis Sativa L. has been studied due to the psychoactive ingredients it contains. Demand for Cannabis oil has shown consistent growth since outbreak of the COVID-19 pandemic, and therefore more studies are required to improve or develop new extraction methods. The purpose of this research was to apply microwave combined with ultrasound technologies to enhance the oil extraction yield without compromising quality. Total phenolic and cannabinoid (THC, CBD) compounds content, yield, and analgesic properties were evaluated in the oil samples. The combination of processes showed higher oil yield (48.93%); however, the oil had lower values of THC content (32.14 mg/ml oil), lower analgesic quality, and low DPPH? (1.39 mg/ml) and ABTS?+ (23.37 mg/ml) scavenging activity, as well as low content of total phenol compounds (13.53 mg GAE/g oil) and high peroxide value (9.09 mEq O2/Kg). The ultrasound process showed 40.36% yield, 1.02 mg/ml DPPH? value, and 2.30 mg/ml ABTS?+, 24.51 mg GAE/oil g total phenolic content, 7.93 meq/ peroxide value, and enhanced analgesic capacity, followed by the values obtained by microwave. The results suggest that ultrasound represents an alternative process to produce Cannabis oil of high quality. In addition, the Cannabis oil had elevated THC content, which could be related to anti-inflammatory capabilities. http://pubs.sciepub.com/jfnr/11/9/6/jfnr-11-9-6.pdf Cannabis sativa L oil cannabinoids ultrasound microwave