eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2023-06-08 11 6 423 435 10.12691/jfnr-11-6-4 JFNR20231164 article Josyanne Ara迆jo Neves 1 Francilene Vieira da Silva 2 H谷lio Barros Fernandes 2 Ana Fl芍via Seraine Cust車dio Viana 2 Josynaria Ara迆jo Neves 2 Celyane Alves Piauilino 2 Benedito Pereira de Sousa Neto 2 Joselma Sousa Lacerda Lopes 2 Aline Jeane Costa Sousa 2 Ma赤sa Campelo de Sousa 2 Lucillia Rabelo de Oliveira Torres 3 Julia L車pez-Hern芍ndez 4 Ana Rodriguez-Bernaldo de Quir車s 4 Raquel Send車n Garcia 4 Rita de C芍ssia Meneses Oliveira menesesoliveira@gmail.com 4 Federal Education Institute, Science and Technology of Maranh?o, Cod車, Brazil Medicinal Plants Research Center, Health Sciences Center, Federal University of Piau赤, Teresina, Brazil Federal Education Institute, Science and Technology of Maranh?o, Caxias, Brazil Analytical Chemistry, Nutrition and Food Science Department, Faculty of Pharmacy, University of Santiago de Compostela, Santiago de Compostela, Spain The gastric ulcer is definited as erosion of the stomach's mucosal wall, which affects over 4.6 million people per year. The objective was to investigate the phenolic profile and the antioxidant activity of Melipona compressipes fasciculata pot-pollen collected at seasons rainy (PPMF1) and dry (PPMF2), as well as the gastroprotective and ulcer healing activities of PPMF2, as PPMF1 did not have a gastroprotective effect in screening protocols. The phenolic profile revealed compounds such as caffeic acid, quercetin, myricetin and naringenin. In models of gastric lesions by ethanol, ischemia/reperfusion (I/R) and acetic acid, PPMF2 reduced the lesion area (at doses 3 to 80 mg/kg, p.o.) and volume at doses (3 to 24 mg/kg, p.o.) with protection at percent 52 to 92%. PPMF2 too, significantly (p<0.05) elevated mucus and non-protein sulfhydryl groups (SH-NP) with an increase of 122% and 92% respectivelly compared to vehicle group, and decreased malonaldehyde (MDA) in rats (36% compared to vehicle group). As for cicatrizant activity in acetic acid-induced ulcer, PPMF2 promoted healing rates until 97%, caused significant microscopic gastric recovery, elevation of SH-NP (103%), attenuation of MDA (84%) levels and improvement of biochemical profile (p<0.05). This is a relevant and pioneering research associating stingless bee (M. compressipes fasciculata) in gastroprotective activity studies. http://pubs.sciepub.com/jfnr/11/6/4/jfnr-11-6-4.pdf stingless bees oxidative stress phenolics gastroprotection pot-pollen