@article{jfnr20231144,
author={{Xie, Chuanqi and Wang, Shufen and Lin, Xinying and Yang, Fan and Hu, Juwu and Wu, Jing and Xiong, Wei and Wu, Lei},
title={Effect of n-Hexane Extract of <i>Camellia Oleifera</i> Fruit Hull on Inflammatory Responses in LPS-Induced RAW264.7 Cells},
journal={Journal of Food and Nutrition Research},
volume={11},
number={4},
pages={301--309},
year={2023},
url={http://pubs.sciepub.com/jfnr/11/4/4},
issn={2333-1240},
abstract={<i>Camellia oleifera</i> fruit hull has a variety of biological activities such as anti-oxidation, lipid regulation, and anti-cancer <i>etc</i>, however the effect of<i> C. oleifera</i> fruit hull on inflammatory responses has not been clearly elucidated. This study aimed to explore the effect of n-hexane extract of<i> C. oleifera</i> fruit hull on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264.7 cells using Griess assay, ELISA, western blotting analysis, PCR and immunofluorescence technology. The results demonstrated that n-hexane extract of<i> C. oleifera</i> fruit hull ameliorated the secretion of nitric oxide (NO), prostaglandin E2 (PGE2), tumour necrosis factor ¦Á (TNF¦Á), interleukin (IL)-1¦Â, and IL-6, inhibited the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2), downregulated the mRNA level of iNOS, COX2, TNF¦Á, IL-1¦Â, and IL-6, and improved the cell viability in LPS-induced RAW264.7 cells. Moreover, the phosphorylation of ERK, P38, and JNK and nuclear translocation of nuclear factor-kappa B (NF-¦ÊB) were restrained by the treatment of n-hexane extract of<i> </i><i>C. oleifera</i> fruit hull in LPS-induced RAW264.7 cells. Taken together, these data imply that n-hexane extract of <i>C. oleifera</i> fruit hull alleviates the inflammatory response by regulating the activation of mitogen-activated protein kinases (MAPKs) and NF-¦ÊB signaling pathways in LPS-induced RAW264.7 cells.},
doi={10.12691/jfnr-11-4-4}
publisher={Science and Education Publishing}
}