@article{jfnr202311312,
author={{Yu, Seok-Yeong and Liu, Zhenhua and Chung, Soonkyu and Kim, Young-Cheul},
title={Sulforaphane (SFN) Exerts Anti-inflammatory Effects on LPS-stimulated RAW 264.7 and Mouse Bone Marrow Driven Macrophages by Modulating p65 Phosphorylation and Histone Acetylation},
journal={Journal of Food and Nutrition Research},
volume={11},
number={3},
pages={269--276},
year={2023},
url={http://pubs.sciepub.com/jfnr/11/3/12},
issn={2333-1240},
abstract={Obesity-induced chronic inflammation contributes to the development of insulin resistance and type 2 diabetes and largely pertains to macrophages, primarily derived from bone marrow, (BMDMs) in insulin-sensitive tissues. Sulforaphane (SFN) is a major bioactive of cruciferous vegetables with a potent anti-inflammatory property. However, potential molecular modes of action remain unclear. The phosphorylation of nuclear factor-kappa B (NF-¦ĘB) and subsequent histone acetylation are critical signaling pathways for the transcription of pro-inflammatory genes in macrophages in obesity. Therefore, we tested our hypothesis that SFN mitigates the expression of pro-inflammatory genes in macrophages by suppressing p65 phosphorylation and histone acetylation using RAW 264.7 cell line and primary mouse BMDMs. In RAW 264.7 macrophages, SFN significantly inhibited the lipopolysaccharide (LPS)-induced pro-inflammatory genes expression, including <i>iNos</i>, <i>Cox-2</i>, <i>Tnf¦Á</i>, <i>Mcp-1</i> and <i>Il-6</i>, and suppressed CD11c immunofluorescence. Moreover, SFN significantly inhibited p65 phosphorylation and acetylation levels of histone H4, while it increased the histone deacetylase 3 (HDAC3) expression. Similarly, SFN inhibited p65 phosphorylation and histone H4 acetylation with HDAC3 expression increased in the BMDMs. These results revealed that SFN exerts anti-inflammatory effects via modulation of p65 phosphorylation and histone acetylation in macrophages. Our work suggests that supplementation of SFN or SFN-containing vegetables may serve as an anti-inflammatory diet component for mitigating obesity-related inflammation and related metabolic disorders.},
doi={10.12691/jfnr-11-3-12}
publisher={Science and Education Publishing}
}