eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2022-06-21 10 6 415 424 10.12691/jfnr-10-6-5 JFNR20221065 article Yoo Jung Jeon 1 Sung Hyun Kim 2 Dong Seok Lee mbdslee@inje.ac.kr 2 2 3 Institute of Digital Anti-aging Healthcare, Graduate School, Inje University, Gimhae 50834, Republic of Korea Department of Smart Foods and Drugs, Graduate School, Inje University, Gimhae 50834, Republic of Korea To investigate the anti-atopic dermatitis (AD) activity of dichloromethane (DCM) fraction of ethanol extracts from Orostachys japonicus (OJ) in AD relating human keratinocytes (HaCaT cells) through down-regulation of a key transcription factor NF-百B involved in skin inflammatory responses. The DCM fraction was obtained from OJ ethanol extracts and used for this study. For anti-AD activity, HaCaT cells that have been induced with IFN-污 and TNF-汐 at the same concentration to produce proinflammatory chemokines were used. As analysis methods, WST assay, chemokines measurement, antibody array, nuclear/cytoplasmic protein separation, and western blotting were used. WST assay showed no toxicity to cell viability. The production levels of chemokines such as MDC, RANTES, IL-8, and TARC decreased in a concentration-dependent manner. Immunoassay through the antibody array showed that the DCM fraction down-regulated the NF-百B (p65) expression. In addition, as a result of western blotting, it was revealed that the translocation of NF-百B (p65) from the cytoplasm into the nucleus decreased. The DCM fraction from OJ ethanol extracts showed significant anti-AD activity through suppression of proinflammatory chemokines causing AD in HaCaT cells via down-regulation of expression and translocation of NF-百B (p65) into the nucleus. http://pubs.sciepub.com/jfnr/10/6/5/jfnr-10-6-5.pdf Orostachys japonicas atopic dermatitis keratinocyte chemokine HaCaT NF-百B (p65)