@article{jfnr20221065,
author={{Jeon, Yoo Jung and Kim, Sung Hyun and Lee, Dong Seok},
title={<i>Orostachys japonicus</i> Suppresses IFN-污 and TNF-&#945; Induced Proinflammatory Chemokines in Atopic Dermatitis Relating HaCaT Cells via Down-regulation of Expression and Translocation of NF-百B},
journal={Journal of Food and Nutrition Research},
volume={10},
number={6},
pages={415--424},
year={2022},
url={http://pubs.sciepub.com/jfnr/10/6/5},
issn={2333-1240},
abstract={To investigate the anti-atopic dermatitis (AD) activity of dichloromethane (DCM) fraction of ethanol extracts from <i>O</i><i>rostachys </i><i>japonicus</i><i> </i>(OJ) in AD relating human keratinocytes (HaCaT cells) through down-regulation of a key transcription factor NF-百B involved in skin inflammatory responses. The DCM fraction was obtained from OJ ethanol extracts and used for this study. For anti-AD activity, HaCaT cells that have been induced with IFN-污 and TNF-汐 at the same concentration to produce proinflammatory chemokines were used. As analysis methods, WST assay, chemokines measurement, antibody array, nuclear/cytoplasmic protein separation, and western blotting were used. WST assay showed no toxicity to cell viability. The production levels of chemokines such as MDC, RANTES, IL-8, and TARC decreased in a concentration-dependent manner. Immunoassay through the antibody array showed that the DCM fraction down-regulated the NF-百B (p65) expression. In addition, as a result of western blotting, it was revealed that the translocation of NF-百B (p65) from the cytoplasm into the nucleus decreased. The DCM fraction from OJ ethanol extracts<i> </i>showed significant anti-AD activity through suppression of proinflammatory chemokines causing AD in HaCaT cells via down-regulation of expression and translocation of NF-百B (p65) into the nucleus.},
doi={10.12691/jfnr-10-6-5}
publisher={Science and Education Publishing}
}